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Methods in Cell Biology

By N.a
  • ISBN Code: : 012820009X
  • Publisher : Academic Press
  • Pages : 194
  • Category : Science
  • Reads : 542
  • Book Compatibility : Pdf, ePub, Mobi, Kindle
  • Pdf : methods-in-cell-biology.pdf

Book Excerpt :

Methods in Cell Biology, Volume 158, the latest release in this series, highlights new advances in the field, with this release covering How to orient cells in micro-cavities for high resolution imaging of cytokinesis and lumen formation, A body-on-a-chip (BOC) system for studying gut-liver interaction, Manipulating cultured mammalian cells for mitosis research, Live-cell FLIM-FRET using a commercially available system, A comparative analysis of methods to measure kinetochore-microtubule attachment stability, A workflow for visualizing human cancer biopsies using large-format electron microscopy, Isolation of stage-specific germ cells using facs in drosophila germarium, Computational analysis of filament polymerization dynamics in cytoskeletal networks, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Updated release includes the latest information in this area of study

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Laboratory Methods in Cell Biology: Imaging

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 0124078079
  • Total of Pages : 464
  • Category : Science
  • Members : 595
  • Pdf File: laboratory-methods-in-cell-biology.pdf

Book Short Summary:

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields

Quantitative Imaging in Cell Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 0124202012
  • Total of Pages : 588
  • Category : Science
  • Members : 817
  • Pdf File: quantitative-imaging-in-cell-biology.pdf

Book Short Summary:

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Methods in Cell Biology

By N.a
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  • Publisher : Academic Press
  • Book Code : 9780080859040
  • Total of Pages : 491
  • Category : Science
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  • Pdf File: methods-in-cell-biology.pdf

Book Short Summary:

Methods in Cell Biology

Expansion Microscopy for Cell Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 0128208082
  • Total of Pages : 356
  • Category : Science
  • Members : 444
  • Pdf File: expansion-microscopy-for-cell-biology.pdf

Book Short Summary:

Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more. Provides the authority and expertise of leading contributors from an international board of authors Represents the latest release in the Methods in Cell Biology series Includes the latest information on Expansion Microscopy for Cell Biology

Methods in Cell Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 9780080859071
  • Total of Pages : 451
  • Category : Science
  • Members : 493
  • Pdf File: methods-in-cell-biology.pdf

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Methods in Cell Biology

Methods in Cell Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 9780080858982
  • Total of Pages : 464
  • Category : Science
  • Members : 276
  • Pdf File: methods-in-cell-biology.pdf

Book Short Summary:

Methods in Cell Biology

Methods in Cell Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 9780080859170
  • Total of Pages : 519
  • Category : Science
  • Members : 756
  • Pdf File: methods-in-cell-biology.pdf

Book Short Summary:

Methods in Cell Biology

Fluorescence Microscopy of Living Cells in Culture, Part B

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 9780080859286
  • Total of Pages : 502
  • Category : Science
  • Members : 677
  • Pdf File: fluorescence-microscopy-of-living-cells-in-culture-part-b.pdf

Book Short Summary:

Fluorescence Microscopy of Living Cells in Culture, Part B

Methods in Cell Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 9780080859118
  • Total of Pages : 421
  • Category : Science
  • Members : 407
  • Pdf File: methods-in-cell-biology.pdf

Book Short Summary:

Methods in Cell Biology

Biophysical Methods in Cell Biology

By N.a
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  • Publisher : Academic Press
  • Book Code : 0128013265
  • Total of Pages : 568
  • Category : Science
  • Members : 128
  • Pdf File: biophysical-methods-in-cell-biology.pdf

Book Short Summary:

This new volume of Methods in Cell Biology looks at methods for analyzing of biophysical methods in cell biology. Chapters cover such topics as AFM, traction force microscopy, digital holographic microscopy, single molecule imaging, video force microscopy and 3D multicolor super-resolution screening Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Methods in Plant Cell Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 9780080859484
  • Total of Pages : 555
  • Category : Science
  • Members : 196
  • Pdf File: methods-in-plant-cell-biology.pdf

Book Short Summary:

Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. This is accompanied by examples of typical results, illustrations, troubleshooting of potential pitfalls, sources of chemicals and equipment, and complete reference lists. Protocols are written to be easily comprehended by beginning research students, but these extensive volumes will also be a valuable addition to the libraries of expert researchers. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants

Methods in Cell Biology

By N.a
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  • Publisher : Academic Press
  • Book Code : 9780080859064
  • Total of Pages : 396
  • Category : Science
  • Members : 732
  • Pdf File: methods-in-cell-biology.pdf

Book Short Summary:

Methods in Cell Biology

Methods in Extracellular Matrix Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 0128122986
  • Total of Pages : 468
  • Category : Science
  • Members : 700
  • Pdf File: methods-in-extracellular-matrix-biology.pdf

Book Short Summary:

Methods in Extra Cellular Matrix, Volume 142, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections devoted to Elastin, Quantification of collagen and elastin, Fibrillins, Lysyl oxidase, Fibulins, Matrilins, Hyaluronic Acid, Small leucine-rich proteoglycans, Syndecans, Fibronectin, SPARC, Thrombospondins, Tenascins, Collagen IV, Multi-photon analysis of ECM, Cell-derived extracellular matrices, Laminins, Fibrillar Collagens, Imaging ECM in developing embryos, Analysis of Matrix Degradation, Ultrastructural analysis of ECM, Versican and Large proteoglycans, and an ECM crosslink analysis. This series covers a wide array of topics about the extracellular matrix, including an understanding of crucial proteins and glycoproteins components of ECM. Contains contributions from experts in the field from across the world Covers a wide array of topics on the extracellular matrix, including an understanding crucial proteins and the glycoproteins components of ECM Includes analysis based topics, such as quantification of collagen and elastin, mulit-photon analysis of ECM and ECM crosslink analysis

Monitoring Vesicular Trafficking in Cellular Responses to Stress

By N.a
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  • Publisher : Academic Press
  • Book Code : 0128235454
  • Total of Pages : 224
  • Category : Science
  • Members : 293
  • Pdf File: monitoring-vesicular-trafficking-in-cellular-responses-to-stress.pdf

Book Short Summary:

Monitoring Vesicular Trafficking in Cellular Responses to Stress, Volume 164 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics. Each chapter is written by an international board of authors. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of Monitoring vesicular trafficking in cellular responses to stress

MCB: CAR T Cells: Development, Characterization and Applications

By Lorenzo Galluzzi,Sheila Spada
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  • Publisher : Academic Press
  • Book Code : 0323855024
  • Total of Pages : 242
  • Category : Science
  • Members : 252
  • Pdf File: mcb.pdf

Book Short Summary:

MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice, Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using ?-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells

Atomic Force Microscopy in Cell Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 0080549446
  • Total of Pages : 415
  • Category : Science
  • Members : 919
  • Pdf File: atomic-force-microscopy-in-cell-biology.pdf

Book Short Summary:

This is the first book to cover the history, structure, and application of atomic force microscopy in cell biology. Presented in the clear, well-illustrated style of the Methods in Cell Biology series, it introduces the AFM to its readers and enables them to tap the power and scope of this technology to further their own research. A practical laboratory guide for use of the atomic force and photonic force microscopes, it provides updated technology and methods in force spectroscopy. It is also a comprehensive and easy-to-follow practical laboratory guide for the use of the AFM and PFM in biological research.

Introduction to Electron Microscopy for Biologists

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 008088816X
  • Total of Pages : 560
  • Category : Science
  • Members : 192
  • Pdf File: introduction-to-electron-microscopy-for-biologists.pdf

Book Short Summary:

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). *Basic techniques in transmission and scanning electron microscopy *Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton *Discussion on electron microscopy of viruses and virus-cell interactions

Cellular Electron Microscopy

By J. Richard McIntosh
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  • Publisher : Elsevier
  • Book Code : 9780080475035
  • Total of Pages : 880
  • Category : Science
  • Members : 883
  • Pdf File: cellular-electron-microscopy.pdf

Book Short Summary:

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. Updates the numerous technological innovations that have improved the capabilities of electron microscopy Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

Cellular and Molecular Methods in Neuroscience Research

By Adalberto Merighi,Giorgio Carmignoto
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Springer Science & Business Media
  • Book Code : 0387224602
  • Total of Pages : 303
  • Category : Medical
  • Members : 996
  • Pdf File: cellular-and-molecular-methods-in-neuroscience-research.pdf

Book Short Summary:

There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

Mitochondria Biology

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 0128202297
  • Total of Pages : 604
  • Category : Science
  • Members : 554
  • Pdf File: mitochondria-biology.pdf

Book Short Summary:

Methods in Cell Biology Volume 155 provides an update on the step-by-step "how-to" methods to study mitochondrial structure, function and biogenesis contained in the first two editions. As in the previous editions, biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls for each method. New chapters in this update include Isolation of Mitochondria and Analysis of Mitochondrial Compartments, Isolation of Mitochondria from Animal Cells and Yeast, Isolation and Characterization of Mitochondria-Associated ER Membranes, Import of Proteins into Mitochondria, Proximity Labeling Methods to Assess Protein-Protein Interactions in Yeast Mitochondria, and more. Provides a step-by-step "cookbook" presentation as written by leaders in the field Covers longstanding methods that have shaped the field Includes the newest technologies and methods

Biomolecular Interactions Part A

By N.a
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  • Publisher : Academic Press
  • Book Code : 0128233524
  • Total of Pages : 368
  • Category : Science
  • Members : 275
  • Pdf File: biomolecular-interactions-part-a.pdf

Book Short Summary:

Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics in cell biology. Each chapter is written by an international board of authors. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Updated release includes the latest information on biomolecular interactions instead of protein-protein interactions

Correlative Light and Electron Microscopy IV

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 0128220597
  • Total of Pages : 456
  • Category : Science
  • Members : 641
  • Pdf File: correlative-light-and-electron-microscopy-iv.pdf

Book Short Summary:

Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more. Contains contributions from experts in the field Covers topics using nano-SIMS and EDX for CLEM Presents recent advances and currently applied correlative approaches Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting Covers CLEM approaches in the context of specific applications Aims to stimulate the use of new combinations of imaging modalities

Methods in Cell-Matrix Adhesion

By Josephine Adams
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  • Publisher : Academic Press
  • Book Code : 0080524966
  • Total of Pages : 461
  • Category : Science
  • Members : 636
  • Pdf File: methods-in-cell-matrix-adhesion.pdf

Book Short Summary:

Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual background for all aspects of cell-matrix adhesion research: the extracellular matrix, adhesion receptors, and the growing number of functional applications of matrix-adhesion in molecular cell biology. Also covered is the purification of the extracellular matrix to functional analyses of cellular responses.

Methods in Cell Physiology

By David M. Prescott
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  • Publisher : Elsevier
  • Book Code : 1483264033
  • Total of Pages : 480
  • Category : Medical
  • Members : 214
  • Pdf File: methods-in-cell-physiology.pdf

Book Short Summary:

Methods in Cell Physiology, Volume I, brings together into one compilation, complete and detailed treatments of a number of widely useful techniques in modern cell biology which have not been published in full form elsewhere in the literature. The presentations in this volume are comprehensive to the extent that they may serve not only as a practical introduction to experimental procedures but they also provide some evaluation of the limitations, potentialities, and current applications of the methods. Only those theoretical considerations needed for proper use of the method are included. The book may have particular usefulness for those working with intact cells, and the first chapters deal with culturing and experimental manipulation of a variety of cell types. There are numerous descriptions of techniques designed for working with single cells and of procedures for studying cellular activities in relation to the cell life cycle. The chapters on autoradiography include detailed treatment of qualitative and quantitative aspects of the method as well as simple procedures for using liquid and dry emulsions.

Correlative Light and Electron MIcroscopy

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 0123914388
  • Total of Pages : 460
  • Category : Science
  • Members : 795
  • Pdf File: correlative-light-and-electron-microscopy.pdf

Book Short Summary:

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods

Methods in Cell Wall Cytochemistry

By K V Krishnamurthy
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : CRC Press
  • Book Code : 1000101843
  • Total of Pages : 336
  • Category : Science
  • Members : 762
  • Pdf File: methods-in-cell-wall-cytochemistry.pdf

Book Short Summary:

Various methodologies designed to study cell walls are compiled in this book. Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.) Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry. Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.

Calcium in Living Cells

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 9780080951225
  • Total of Pages : 328
  • Category : Science
  • Members : 294
  • Pdf File: calcium-in-living-cells.pdf

Book Short Summary:

Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging

Plant Cell Culture Protocols

By Víctor M. Loyola-Vargas,Felipe Vázquez-Flota
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Springer Science & Business Media
  • Book Code : 1592599591
  • Total of Pages : 394
  • Category : Science
  • Members : 248
  • Pdf File: plant-cell-culture-protocols.pdf

Book Short Summary:

A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Nuclear Structure and Function

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Elsevier
  • Book Code : 0080859518
  • Total of Pages : 625
  • Category : Science
  • Members : 946
  • Pdf File: nuclear-structure-and-function.pdf

Book Short Summary:

This volume is a comprehensive guide to the methodologies used in the study of structural domains of cell nuclei. The text covers chromatin, the karyoskeleton, the soluble domain, and the nucleolus. It details methods that are used to isolate components from these domains and techniques used to assemble and disassemble nuclear elements. There is also coverage of three-dimensional mapping and localization of nuclear processes. Key Features * Provides a practical laboratory guide for studying cell nuclei * Includes comprehensive and easy-to-follow protocols

A Practical Guide to the Study of Calcium in Living Cells

By N.a
  • File : Pdf, ePub, Mobi, Kindle
  • Publisher : Academic Press
  • Book Code : 9780080859385
  • Total of Pages : 368
  • Category : Science
  • Members : 931
  • Pdf File: a-practical-guide-to-the-study-of-calcium-in-living-cells.pdf

Book Short Summary:

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin

Stochastic Processes in Cell Biology

By Paul C. Bressloff
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  • Publisher : Springer
  • Book Code : 3319084887
  • Total of Pages : 679
  • Category : Mathematics
  • Members : 706
  • Pdf File: stochastic-processes-in-cell-biology.pdf

Book Short Summary:

This book develops the theory of continuous and discrete stochastic processes within the context of cell biology. A wide range of biological topics are covered including normal and anomalous diffusion in complex cellular environments, stochastic ion channels and excitable systems, stochastic calcium signaling, molecular motors, intracellular transport, signal transduction, bacterial chemotaxis, robustness in gene networks, genetic switches and oscillators, cell polarization, polymerization, cellular length control, and branching processes. The book also provides a pedagogical introduction to the theory of stochastic process – Fokker Planck equations, stochastic differential equations, master equations and jump Markov processes, diffusion approximations and the system size expansion, first passage time problems, stochastic hybrid systems, reaction-diffusion equations, exclusion processes, WKB methods, martingales and branching processes, stochastic calculus, and numerical methods. This text is primarily aimed at graduate students and researchers working in mathematical biology and applied mathematicians interested in stochastic modeling. Applied probabilists and theoretical physicists should also find it of interest. It assumes no prior background in statistical physics and introduces concepts in stochastic processes via motivating biological applications. The book is highly illustrated and contains a large number of examples and exercises that further develop the models and ideas in the body of the text. It is based on a course that the author has taught at the University of Utah for many years.